The Effect of Iron Overload on Osteoblast Function in Cell Culture
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Amy K Kilbarger (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- Deborah Kipp
Abstract: Human, animal, and cell culture studies support the relationship between iron overload and lowered bone mineral density. The hypothesis of the studies in this thesis was that: iron accumulates intracellularly and that this accumulation results in increased iron levels that alter iron-regulatory proteins and also maximally suppress osteoblast maturation and function. Osteoblast-like cells isolated from fetal rat calvaria were treated with ferrous sulfate (FeSO4) (0-10 µM). Intracellular iron concentration was increased 25-fold greater than control upon 5 µM FeSO4 exposure. Transferrin receptor and ferritin light-chain protein and gene expression were altered and osteoblast phenotypes were markedly suppressed with excessive FeSO4 treatment. Excessive FeSO4 treatment resulted in high intracellular iron accumulation, alterations in key iron-regulated gene and protein expression, and the suppression osteoblast maturation and function in a concentration-dependent and time-dependent manner. These results provide the basis for evaluation of mechanisms by which iron overload alters osteoblast maturation and function.
The Effect of Iron Overload on Osteoblast Function in Cell Culture
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Created on 12/1/2007
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Additional Information
- Publication
- Thesis
- Language: English
- Date: 2007
- Keywords
- Osteoblast, Bone, Iron, Overload, Nutrition
- Subjects
- Iron $x Pathophysiology.
- Bones $x Growth $x Physiology.
- Iron $x Toxicology.
- Bones $x Growth $x Molecular aspects.
- Bone densitometry.