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The Effect of Iron Overload on Osteoblast Function in Cell Culture

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Amy K Kilbarger (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Deborah Kipp

Abstract: Human, animal, and cell culture studies support the relationship between iron overload and lowered bone mineral density. The hypothesis of the studies in this thesis was that: iron accumulates intracellularly and that this accumulation results in increased iron levels that alter iron-regulatory proteins and also maximally suppress osteoblast maturation and function. Osteoblast-like cells isolated from fetal rat calvaria were treated with ferrous sulfate (FeSO4) (0-10 µM). Intracellular iron concentration was increased 25-fold greater than control upon 5 µM FeSO4 exposure. Transferrin receptor and ferritin light-chain protein and gene expression were altered and osteoblast phenotypes were markedly suppressed with excessive FeSO4 treatment. Excessive FeSO4 treatment resulted in high intracellular iron accumulation, alterations in key iron-regulated gene and protein expression, and the suppression osteoblast maturation and function in a concentration-dependent and time-dependent manner. These results provide the basis for evaluation of mechanisms by which iron overload alters osteoblast maturation and function.

Additional Information

Publication
Thesis
Language: English
Date: 2007
Keywords
Osteoblast, Bone, Iron, Overload, Nutrition
Subjects
Iron $x Pathophysiology.
Bones $x Growth $x Physiology.
Iron $x Toxicology.
Bones $x Growth $x Molecular aspects.
Bone densitometry.