Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Shaw M. Akula (Creator)

De Chen (Creator)

Ossie F. Dyson (Creator)

Patrick W. Ford (Creator)

Yong-qing Li (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Kaposi's sarcoma-associated herpesvirus (KSHV) has both latent and lytic phases of replication. The molecular switch that triggers a reactivation is still unclear. Cells from S phase of cell cycle provide apt conditions for an active reactivation. In order to specifically delineate the Raman spectra of cells supporting KSHV reactivation we followed a novel approach where cells were sorted based on the state of infection (latent Vs lytic) by a flow cytometer and then analyzed by the Raman tweezers. The Raman bands at 785 813 830 1095 and 1128 cm-1 are specifically altered in cells supporting KSHV reactivation. These 5 peaks make up the Raman fingerprint of cells supporting KSHV reactivation. The physiological relevance of the changes in these peaks with respect to KSHV reactivation is discussed in the following report. Originally published Journal of Cellular and Molecular Medicine Vol. 13 No. 8b Aug 2009

Additional Information

Publication

Other

Journal of Cellular and Molecular Medicine. 13:8b(August 2009) p. 1920-1932.

Language: English

Date: 2011

Keywords

KSHV, Latency, S phase, Raman tweezers, Reactivation

Email this document to