Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene cblA encoding the species-specific beta-lactamase.

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Tamara K. Bennett (Creator)

Anita C. Parker (Creator)

C. Jeffrey Smith (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: The gene cbl encoding the species-specific clavulanate-susceptible endogenous cephalosporinase was cloned from Bacteroides uniforinis WAL-7088. The nucleotide sequence was determined and the cbl structural gene was found to be 891 nucleotides with a 48% G+C composition which is similar to that of the B. uniformis genome. The cbhi open reading frame encoded an Ambler class A B-lactamase polypeptide precursor of 296 amino acid residues with a predicted molecular weight of 33 450. A -lactamase-deficient B. uniformis mutant with increased -lactam susceptibility was constructed by insertional inactivation of the chromosomal gene. This mutant was complemented by plasmids bearing the cblA gene and the resulting strains were resistant to cephaloridine and had a P-lactamase that comigrated with the parental j-lactamase on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (30 500 Da) and in isoelectric focusing gels (pl 4.6) confirming a role for this P-lactamase in resistance. Originally published Antimicrobial Agents and Chemotherapy Vol. 38 No. 8 Aug 1994

Additional Information

Publication

Other

Antimicrobial Agents and Chemotherapy. 38:8(August 1994) p. 1711-1715.

Language: English

Date: 2011

Keywords

beta-lactamase, cephalosporinase gene, cephaloridine resistance

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