MCF-7 breast cancer cells transfected with protein kinase C-alpha exhibit altered expression of other protein kinase C isoforms and display a more aggressive neoplastic phenotype.
- ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)
- Winifred O. Bryant (Creator)
- Paul P. Cook (Creator)
- James deVente (Creator)
- Donald J. Fletcher (Creator)
- Jerry L. Hooker (Creator)
- Cynthia A. Kukoly (Creator)
- Peter J. Parker (Creator)
- Karla J. Posekany (Creator)
- D. Kirk Ways (Creator)
- Institution
- East Carolina University (ECU )
- Web Site: http://www.ecu.edu/lib/
Abstract: Increased protein kinase C (PKC) activity in malignant breast tissue and positive correlations between PKC activity and expression of a more aggressive phenotype in breast cancer cell lines suggest a role for this signal transduction pathway in the pathogenesis and/or progression of breast cancer. To examine the role of PKC in the progression of breast cancer human MCF-7 breast cancer cells were transfected with PKC-a and a group of heterogenous cells stably over expressing PKC-a were isolated (MCF-7-PKC- a).MCF-7-PKC-a cells expressed five fold higher levels of PKC-a as compared to parental or vector-transfected MCF- 7 cels. MCF-7-PKC-a cells also displayed a substantial increase in endogenous expression of PKC-8 and decreases in expression of the novel 6- and q-PKC isoforms. MCF-7-PKC-a cells displayed an enhanced proliferative rate anchorage-independent growth dramatic morphologic alterations including loss of an epithelioid appearance and increased tumorigenicity in nude mice. MCF-7-PKC- a cells exhibited a significant reduction in estrogen receptor expression and decreases in estrogen-dependent gene expression. These findings suggest that the PKC pathway may modulate progression of breast cancer to a more aggressive neoplastic process. Originally published Journal of Clinical Investigation Vol. 95 No. 4 Apr 1995
Additional Information
- Publication
- Other
- Journal of Clinical Investigation. 95:4(April 1995) p. 1906-1915.
- Language: English
- Date: 2011
- Keywords
- PKC isoforms, vimentin, estrogen receptor, anchorage independent, tumorigenicity
| Title | Location & Link | Type of Relationship |
| MCF-7 breast cancer cells transfected with protein kinase C-alpha exhibit altered expression of other protein kinase C isoforms and display a more aggressive neoplastic phenotype. | http://hdl.handle.net/10342/3403 | The described resource references, cites, or otherwise points to the related resource. |