HuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytes

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Melinda Carver (Creator)

Heath Jones (Creator)

Phillip H. Pekala (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: HuR is a ligand for nuclear mRNAs containing adenylate-uridylate rich elements in the 3'- untranslated region. Once bound to the mRNA HuR is recognized by adapter proteins which then facilitate nuclear export of the complex. In the cytosol HuR is thought to function to control stability and translation of its ligand message. In the 3T3-L1 cells HuR is constitutively expressed and localized predominantly to the nucleus in the preadipocytes. However within 30 min of exposure to the differentiation stimulus the HuR content in the cytosol increases consistent with HuR regulating the availability of relevant mRNAs for translation. Using in vitro RNA gel shifts we have demonstrated that the C/EBPß message is a ligand for HuR and that the single binding site is an adenylate-uridylate rich element in the 3'untranslated region. Originally published Biochemical and Biophysical Research Communications Vol. 355 No. 1 Mar 2007

Additional Information

Publication

Other

Biochemical and Biophysical Research Communications. 355:1(March 2007) p. 217-220.

Language: English

Date: 2011

Keywords

CCAAT enhancer binding protein beta, HuR, elav, 3T3-L1, adipocytes, differentiation

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