Plasma protein carbonyl response to increasing exercise duration in aerobically trained men and women

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Laurie Wideman, Safrit-Ennis Distinguished Professor (Creator)
Leslie Consitt (Contributor)
Paul Davis, Associate Professor (Contributor)
The University of North Carolina at Greensboro (UNCG )
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Abstract: The purpose of this study was to determine the effect of aerobic exercise duration on plasma protein carbonyl concentrations, a marker of protein oxidation, in aerobically trained men and women. Eight men (age: 27 ± 4 years, V·O2peak: 4.09 ± 0.26 L · min-1; mean ± SD) and 7 women (age: 27 ± 6 years, V·O2peak: 2.33 ± 0.24 L · min-1) exercised on an electrically-braked cycle ergometer at 70 % V·O2peak for 30, 60 or 120 minutes on three separate days. Plasma samples collected before and immediately, 30- and 60-minutes post-exercise were analyzed for protein carbonyls. Mean oxygen uptake was greater for men in all conditions (2.75 ± 0.03 L · min-1; 38 ± 0.43 ml · kg-1 · min-1) compared to women (1.57 ± 0.03 L · min-1; 24.1 ± 0.47 ml · kg-1 · min-1). Total work performed during the exercise sessions was also greater for men than for women during the 30 (368 ± 11 versus 223 ± 7 kJ), 60 (697 ± 17 versus 423 ± 18 kJ), and 120-minute conditions (1173 ± 44 versus 726 ± 28 kJ) (Mean ± SEM). Although these comparisons were significant (p < 0.0001), sex differences in total work performed and mean V·O2 did not result in sex differences in protein carbonyls. However, a condition by time interaction was observed with greater post-exercise values following the 120-minute condition compared to both the 30- and 60-minute conditions. Protein carbonyl concentration was greatest immediately post-exercise for both men and women and generally declined in a linear trend through one hour of recovery. These data suggest that protein carbonyl concentration is elevated by cycling exercise performed at 70 % V·O2peak, is greater following longer duration rides, begins to recover within one hour following exercise, and is not different between men and women.

Additional Information

International Journal of Sports Medicine 28(1):21-25.
Language: English
Date: 2007
Oxidative stress, Cycling, Reactive oxygen species

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