Metabolomics approach to assessing plasma 13- and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Dr.. David Nieman, Director (Creator)
Andrew Shanely Ph.D, Associate professor (Creator)
Institution
Appalachian State University (ASU )
Web Site: http://www.library.appstate.edu/

Abstract: Bioactive oxidized linoleic acid metabolites (OXLAMs) include 13- and 9-hydroxy-octadecadienoic acid (13-HODE + 9-HODE) and have been linked to oxidative stress, in?ammation, and numerous pathological and physiological states. The purpose of this study was to measure changes in plasma 13-HODE + 9-HODE following a 75-km cycling bout and identify potential linkages to linoleate metabolism and established biomarkers of oxidative stress (F2-isoprostanes) and in?ammation (cytokines) using a metabolomics approach. Trained male cyclists (N ?? 19, age 38.0 + 1.6 yr, wattsmax 304 +10.5) engaged in a 75-km cycling time trial on their own bicycles using electromagnetically braked cycling ergometers (2.71 + 0.07 h). Blood samples were collected preexer-cise, immediately post-, 1.5 h post-, and 21 h postexercise, and analyzed for plasma cytokines (IL-6, IL-8, IL-10, tumor necrosis factor-??, monocyte chemoattractant protein-1, granulocyte colony-stimulating factor), F2-isoprostanes, and shifts in metabolites using global metabolomics procedures with gas chromatography mass spec-trometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS). 13-HODE +9-HODE increased 3.1-fold and 1.7-fold immediately post- and 1.5 h postexercise (both P <0.001) and returned to preexercise levels by 21-h postexercise. Post-75-km cy-cling plasma levels of 13-HODE ?? 9-HODE were not signi?cantly correlated with increases in plasma cytokines but were positively correlated with postexercise F2-isoprostanes (r =0.75, P < 0.001), linoleate (r = 0.54, P = 0.016), arachidate (r = 0.77, P < 0.001), 12,13-dihydroxy-9Z-octadecenoate (12,13-DiHOME) (r = 0.60, P = 0.006), dihomo-linolenate (r =0.57, P = 0.011), and adrenate (r = 0.56, P = 0.013). These ?ndings indicate that prolonged and intensive exercise caused a transient, 3.1-fold increase in the stable linoleic acid oxidation product 13-HODE + 9-HODE and was related to increases in F2-isoprostanes, linoleate, and fatty acids in the linoleate conver-sion pathway. These data support the use of 13-HODE + 9-HODE as an oxidative stress biomarker in acute exercise investigations

Additional Information

Publication
David C. Nieman, R. Andrew Shanely, Beibei Luo, Mary Pat Meaney, Dustin A. Dew and Kirk L. Pappan (2014) "Metabolomics approach to assessing plasma 13- and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling" American Journal of Physiology-Regulatory, integrative and comparative Physiology Version of Record Available @ (doi:10.1152/ajpregu.00092.2014.)
Language: English
Date: 2014
Keywords
oxidative stress, inflammation, linoleate, metabolites

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