Identification and Localization of H+-ATPase, NHE2 and NHE3 in the gills of the southern Appalachian brook trout, Salvelinus fontinalis
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Brian Patrick Mikeworth (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
- Advisor
- Susan Edwards
Abstract: Southern brook trout (Salvelinus fontinalis) native to the Great Smoky Mountains are found in headwater streams. These habitats experience increased instances of stream acidification, lowering stream pH on average ~1.0 pH unit. It is well documented that lowering environmental pH results in fish experiencing systemic acidosis. We hypothesize that during acidification events, brook trout suffer acid/base perturbation. Previous studies on other species of freshwater fishes have demonstrated an alteration in the relative expression of H+-ATPase, NHE2 and NHE3 in gill tissue during pH disturbances. Due to a lack of physiological information available, the mechanism utilized by S. fontinalis to regulate systemic pH is unknown. We hypothesized that S. fontinalis will utilize H+-ATPase, NHE2 and NHE3 to excrete excess protons when experiencing episodic stream acidification. To date, we cloned ORFs for H+-ATPase, NHE2, and NHE3 which are homologous to rainbow trout (87, 94 and 92% identical respectively). Immunohistochemical analysis demonstrated H+-ATPase, NHE2 and NHE3 expression is localized to the apical membrane and sub-apical regions of the MRCs in gill epithelia. Also, the relative expression of H+-ATPase decreased along an elevation gradient, whereas NHE3 does not.
Identification and Localization of H+-ATPase, NHE2 and NHE3 in the gills of the southern Appalachian brook trout, Salvelinus fontinalis
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Created on 7/24/2012
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Additional Information
- Publication
- Thesis
- Mikeworth, B.P. (2012). Identification and Localization of H+-ATPase, NHE2 and NHE3 in the gills of the southern Appalachian brook trout, Salvelinus fontinalis. Unpublished master’s thesis. Appalachian State University, Boone, NC.
- Language: English
- Date: 2012
- Keywords
- Brook trout, Acid-base regulation, Osmoregulation, Mitochondrion-rich cells, Episodic stream acidification, Great Smoky Mountains National Park