Microbial Communication With Extracellular Vesicles In Interspecies And Interkingdom Interactions
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- William Robert Hardin (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
- Advisor
- Rachel Bleich
Abstract: Microbiomes of the soil and gut form a complex community of bacteria, fungi, viruses, and host cells. Housing many times more genetic material than the host genome and a vast array of bioactive compounds. Understanding the microbiome and the interactions within could prove useful in improving agricultural practices and human health. Transportation of bacterial molecules to more distant cells, tissues, and locations to influence host functions has been attributed to extracellular vesicles (EVs). EVs consist of small molecules, RNAs, and proteins secreted by all kinds of cells and bound by the parent cell's outer membrane. They act as cellular “packages”, housing and transporting compounds for interactions between cells and host structures. EV-mediated changes to host and bacterial functions in both gut and plant models is not well-characterized. The objectives of this research are to 1: isolate EVs from Pseudomonas fluorescens and Saccharomyces cerevisiae, 2: to understand their impact on the growth and soil microbiome composition of Arabidopsis thaliana, and 3: to quantify changes in yield of EVs secreted by Escherichia coli (E. coli) and Enterococcus. The results will help elucidate the role of EVs in cellular communication by bacteria in gut and soil microbiomes.
Microbial Communication With Extracellular Vesicles In Interspecies And Interkingdom Interactions
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Created on 1/30/2024
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Additional Information
- Publication
- Thesis
- Hardin, W. (2023). Microbial Communication With Extracellular Vesicles In Interspecies And Interkingdom Interactions. Unpublished Master’s Thesis. Appalachian State University, Boone, NC.
- Language: English
- Date: 2023
- Keywords
- Extracellular Vesicles, Interspecies Interactions, Interkingdom Interactions, Rhizosphere Microbiome, Inflammatory Bowel Disease