Identification and Developmental Expression of the Zebrafish ZGC:154061 Gene, a Conserved Yet Uncharacterized Maternally Expressed MEIS2 Linked Gene

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Brandon Scott Carpenter (Creator)
Institution
Appalachian State University (ASU )
Web Site: http://www.library.appstate.edu/
Advisor
Ted Zerucha

Abstract: We have identified a novel and previously undescribed gene, zgc:154061, located directly downstream of the zebrafish meis2.2 gene. We have identified putative orthologs of this gene in all animals that we have been able to examine. The zgc:154061 gene and its vertebrate orthologs are organized in a convergently transcribed manner with respect to the Meis2 gene in all species we have examined (meis2.2 in teleosts). It appears that the homologs of Meis and zgc:154061 are also linked in amphioxus and sea urchins but that this linkage is not present in urochordates, nor in protostomes. During zebrafish development, transcripts of zgc:154061 are observed in every cell of the embryo from the earliest stage through the shield stage indicating this gene is a maternal transcript since its expression precedes the activation of the zygotic genome at the midblastula transition. Expression of zgc:154061 gradually decreases from its peak value at 0 hpf until 8 hpf and then is observed to be activated again at 12 hpf throughout the neural tube before becoming restricted to the retina and tectum opticum by 48 hpf.

Additional Information

Publication
Thesis
Carpenter, B.S. (2010). Identification and Developmental Expression of the Zebrafish ZGC:154061 Gene, a Conserved Yet Uncharacterized Maternally Expressed MEIS2 Linked Gene. Unpublished master's thesis. Appalachian State University, Boone, NC.
Language: English
Date: 2010
Keywords
Meis, zgc:154061, Danio rerio, maternal expression, neural tube, branchial arche