Effects Of A Naturally Derived Lipid On Cell Viability And Inflammatory Signaling In The Presence Of Snake Venom
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Clay Jordan Langfeldt (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
- Advisor
- Mark Venable
Abstract: A lipid extract has demonstrated the ability to inhibit the activity of phospholipase A2 (PLA2) found in the snake venom of the Viperidae family. This research focused on studying the effect this lipid would have in cellular systems through viability and inflammatory endpoints. Cellular models were first established with baselines in venom concentrations, lipid concentrations and time. Addition of the lipid extract at high levels, surprisingly reduced viability when in the presence of the venom. This effect was present in groups pre-incubated with the inhibitor as well as when venom was pre-incubated with the inhibitor. The amount of inhibitor (2.5 nmols) added was much larger than that of cellular phospholipids (0.4 nmols) in cell assays and so smaller amounts (0.5-0.05 nmols) were tested demonstrating no significant loss of viability but also no protection of cells. Inflammatory studies focused on the production of eicosanoids. Prostaglandin E2 and leukotriene B4 are two eicosanoids produced through different pathways from arachidonic acid metabolism following phospholipase A2 action. Concentration and time studies were carried out with the prostaglandin E2 but showed no inhibition by the addition of the inhibitor. Concentration and time trial studies of leukotriene B4 demonstrated the lipoxygenase pathway was stimulated and increased production of leukotriene B4. While venom did increase the concentration of leukotriene B4 there was no observed reduction with the introduction of the inhibitor.
Effects Of A Naturally Derived Lipid On Cell Viability And Inflammatory Signaling In The Presence Of Snake Venom
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Created on 3/15/2023
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Additional Information
- Publication
- Thesis
- Langfeldt, C. (2022). Effects Of A Naturally Derived Lipid On Cell Viability And Inflammatory Signaling In The Presence Of Snake Venom. Unpublished Master’s Thesis. Appalachian State University, Boone, NC.
- Language: English
- Date: 2022
- Keywords
- Biology, Biochemistry, Lipid, Venom, Phospholipase