B Cell Production Of Tumor Necrosis Factor In Response To Pneumocystis murina Infection In Mice
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Dr.. Michael Opata, Assistant Professor (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
Abstract: Pneumocystis species are opportunistic fungal pathogens that induce tumor necrosis factor (TNF) production by alveolar macrophages. Here we report that B cells from the draining lymph nodes as well as lung CD4(+) T cells are important producers of TNF upon Pneumocystis murina infection. To determine the importance of B cell-derived TNF in the primary response to P. murina, we generated bone marrow chimeras whose B cells were unable to produce TNF. The lung P. murina burden at 10 days postinfection in TNF knockout (TNFKO) chimeras was significantly higher than that in wild-type (WT) chimeras, which corresponded to reduced numbers of activated CD4(+) T cells in the lungs at this early time point. Furthermore, CD4(+) T cells isolated from P. murina-infected TNFKO chimeras were unable to stimulate clearance of P. murina upon adoptive transfer to recombinase-deficient (RAG1KO) hosts. Together, these data indicate that B cell-derived TNF plays an important function in promoting CD4(+) T cell expansion and production of TNF and facilitating protection against P. murina infection.
B Cell Production Of Tumor Necrosis Factor In Response To Pneumocystis murina Infection In Mice
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Created on 1/23/2023
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Additional Information
- Publication
- Opata, M. M., Ye, Z., Hollifield, M., & Garvy, B. A. (2013). B cell production of tumor necrosis factor in response to Pneumocystis murina infection in mice. Infection and Immunity, 81(11), 4252–4260. Publisher version of record available at: https://doi.org/10.1128/IAI.00744-13
- Language: English
- Date: 2013
- Keywords
- Tumor Necrosis Factor-alpha/genetics, B-Lymphocytes, Pneumocystis