B Lymphocytes Are Required During The Early Priming Of CD4 T Cells For Clearance Of Pneumocystis Infection In Mice
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Dr.. Michael Opata, Assistant Professor (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
Abstract: B cells play a critical role in the clearance of Pneumocystis. In addition to production of Pneumocystis-specific Abs, B cells are required during the priming phase for CD4+ T cells to expand normally and generate memory. Clearance of Pneumocystis was found to be dependent on Ag specific B cells and on the ability of B cells to secrete Pneumocystis-specific Ab, as mice with B cells defective in these functions or with a restricted BCR were unable to control Pneumocystis infection. Because Pneumocystis-specific antiserum was only able to partially protect B cell–deficient mice from infection, we hypothesized that optimal T cell priming requires fully functional B cells. Using adoptive transfer and B cell depletion strategies, we determined that optimal priming of CD4 + T cells requires Bcells during the first 2–3 d of infection and that this was independent of the production of Ab. T cells that were removed from Pneumocystis-infected mice during the priming phase were fully functional and able to clear Pneumocystis infection upon adoptive transfer into Rag12/2 hosts, but this effect was ablated in mice that lacked fully functional B cells. Our results indicate that T cell priming requires a complete environment of Ag presentation and activation signals to become fully functional in this model of Pneumocystis infection.
B Lymphocytes Are Required During The Early Priming Of CD4 T Cells For Clearance Of Pneumocystis Infection In Mice
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Created on 1/23/2023
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Additional Information
- Publication
- Opata MM, Hollifield ML, Lund FE, et al. B Lymphocytes Are Required during the Early Priming of CD4+ T Cells for Clearance of Pneumocystis Infection in Mice. Journal of immunology (Baltimore, Md : 1950). 2015;195(2):611-620. doi:10.4049/jimmunol.1500112. Publisher version of record available at: https://doi.org/10.4049/jimmunol.1500112
- Language: English
- Date: 2015
- Keywords
- Immunologic Memory, Antibodies, Pneumocystis,
Adoptive Transfer