Transcriptional Regulation Of The Meis2 Locus

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Megan Daley Tennant (Creator)
Appalachian State University (ASU )
Web Site:
Ted Zerucha

Abstract: The expression patterns of meis2a and zgc:154061 as well as the expression of a reporter transgene directed by a highly conserved non-coding element, m2de1, in zebrafish, exhibit overlap in expression, specifically around the eye and brain. We hypothesize that this shared expression and conserved genomic organization are due to meis2a and zgc:154061 sharing m2de1. To test whether m2de1 is involved in directing the expression of either of these genes, a portion of m2de1 was excised from the zebrafish genome via the CRISPR/Cas9 system. Embryos lacking this portion of m2de1 displayed markedly reduced meis2a expression in the forebrain and optic tectum and lacked expression in the cerebellum and rhombencephalon, as compared to wildtype embryos. The change in meis2a expression in mutant embryos suggests that m2de1 is an important player in directing the expression of meis2a. The role that m2de1 plays in directing zgc:154061 expression is less clear however this study is revealing important information about the regulation of the Meis genes in development.

Additional Information

Tennant, M. (2018). "Transcriptional Regulation Of The Meis2 Locus." Unpublished Master’s Thesis. Appalachian State University, Boone, NC.
Language: English
Date: 2018
Meis2, Zgc:154061, m2de1, CRISPR, Zebrafish

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