Methanobacterium Paludis Sp. Nov. And A Novel Strain Of Methanobacterium Lacus Isolated From Northern Peatlands
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Suzanna Brauer Ph.D., Associate Professor (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
Abstract: Two mesophilic, hydrogenotrophic methanogens, designated strains SWAN1T and AL-21, were isolated from two contrasting peatlands: a near circumneutral temperate minerotrophic fen in New York State, USA, and an acidic boreal poor fen site in Alaska, USA, respectively. Cells of the two strains were rod-shaped, non-motile, stained Gram-negative and resisted lysis with 0.1 % SDS. Cell size was 0.6 1.5–2.8 mm for strain SWAN1T and 0.45–0.85 1.5–35 mm for strain AL-21. The strains used H2/CO2 but not formate or other substrates for methanogenesis, grew optimally around 32–37 6C, and their growth spanned through a slightly low to neutral pH range (4.7–7.1). Strain AL-21 grew optimally closer to neutrality at pH 6.2, whereas strain SWAN1T showed a lower optimal pH at 5.4–5.7. The two strains were sensitive to NaCl with a maximal tolerance at 160 mM for strain SWAN1T and 50 mM for strain AL-21. Na2S was toxic at very low concentrations (0.01–0.8 mM), resulting in growth inhibition above these values. The DNA G+C content of the genomes was 35.7 mol% for strain SWAN1T and 35.8 mol% for strain AL-21. Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains are members of the genus Methanobacterium. Strain SWAN1T shared 94–97 % similarity with the type strains of recognized species of the genus Methanobacterium, whereas strain AL-21 shared 99 % similarity with Methanobacterium lacus 17A1T. On the basis of phenotypic, genomic and phylogenetic characteristics, strain SWAN1T (5DSM 25820T5JCM 18151T) is proposed as the type strain of a novel species, Methanobacterium paludis sp. nov., while strain AL-21 is proposed as a second strain of Methanobacterium lacus.
Methanobacterium Paludis Sp. Nov. And A Novel Strain Of Methanobacterium Lacus Isolated From Northern Peatlands
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Additional Information
- Publication
- Suzanna L. Brauer, Hinsby Cadillo-Quiroz, Noah Goodson, Joseph B. Yavitt & Stephen H. Zinder (2014) "Methanobacterium Paludis Sp. Nov. And A Novel Strain Of Methanobacterium Lacus Isolated From Northern Peatlands" International Journal of Systematic and Evolutionary Microbiology volume 64 pp. 1473-1480 [10.1099/ijs.0.059964-0] Version of Record Available From www.webofknowledge.com
- Language: English
- Date: 2014
- Keywords
- ACIDIC PEAT BOG, NEW-YORK-STATE, HYDROGENOTROPHIC METHANOGEN, ACIDIPHILIC METHANOGEN, MINEROTROPHIC FEN, PH VALUES, GEN. NOV., BACTERIA, COMMUNITIES, POPULATIONS