Effects of vitamin E deficiency on fatigue and muscle contractile properties

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Andrew Shanely Ph.D, Associate Professor (Creator)
Institution
Appalachian State University (ASU )
Web Site: https://library.appstate.edu/

Abstract: Radical-mediated oxidative damage of skeletal muscle membranes has been implicated in the fatigue process. Vitamin E (VE) is a major chain breaking an-tioxidant that has been shown to reduce contraction-mediated oxidative damage. We hypothesized that VE de?ciency would adversely a?ect muscle contractile function, resulting in a more rapid development of muscular fatigue during exercise. To test this postulate, rats were fed either a VE-de?cient (EDEF) diet or a control (CON) diet containing VE. Following a 12-week feeding period, animals were anesthetized and mechan-ically ventilated. Muscle endurance (fatigue) and con-tractile properties were evaluated using an in situ preparationof the tibialis anterior (TA) muscle. Con-tractile properties of the TA muscle were determined before and after a fatigue protocol. The muscle fatigue protocol consisted of 60 min of repetitive contractions (250 ms trains at 15 Hz; duty cycle=11%) of the TA muscle. Prior to the fatigue protocol, no signi?cant di?erences existed in the force-frequency curves between EDEF and CON animals. At the completion of the fa-tigue protocol, muscular force productionwas signi?-cantly (P<0.05) lower inthe EDEF group (reduced by 69%) compared to CON group (reduced by 38%). Following the fatigue protocol, a right shift existed in the force-frequency curve at low stimulation frequencies ( £ 40 Hz) inthe EDEF animals compared to the CON animals (P<0.05). The stimulated and the contralateral TA muscle from the EDEF animals had signi?cantly higher markers of lipid peroxidationcompared to the same muscles inthe CON animals (P<0.05). These data support the hypothesis that VE de?ciency impairs muscular endurance and alters muscle contractile prop-erties following a prolonged series of contractions.

Additional Information

Publication
Je? S. Coombes, Benjamin Rowell, Stephen L. Dodd, Haydar A. Demirel, Hisashi Naito,R. Andrew Shanely & Scott K.Powers (2002) "Effects of vitamin E de?ciency on fatigue and muscle contractile properties" European Journal of Applied Physiology #87 pp.272-277 Version of Record Available @ (DOI 10.1007/s00421-002-0631-3)
Language: English
Date: 2002
Keywords
Oxidative damage, Antioxidants, Endurance exercise , Redox status

Email this document to