Sixteen Polymorphic Microsateliate Markers For A Federally Threatened Species, Hexastylis Naniflora (Aristolochiaceae), And Co-Occurring Congeners

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Matt Estep, Assistant Professor (Creator)
Institution
Appalachian State University (ASU )
Web Site: https://library.appstate.edu/

Abstract: Premise of study: Twenty mierosalellite loci were developed for the federally threatened species Hexastylis nanillor (Aristolochiaceae) to examine genetic diversity and to distinguish this species from co-occurring congeners, H. heterophylla and H. minor. Methods and Results: Next-generation sequencing approaches were used to identify microsatellite loci and design primers. One hundred fifty-two primer pairs were screened for repeatability, and 20 of these were further characterized for polymorphism. In H. naniflora, the number of alleles identified for polymorphic loci ranged from two to 23 (mean -8.8), with a mean heterozygosit y of 0.39. Conclusions: These 16 polymorphic primers for H. naniflora will be useful tools in species identification and quantifying genelic diversity within the genus.

Additional Information

Publication
Jacqueline W. Hamstead, Brandon L. Snider , Robyn Oaks , Evan Fitzgerald, Jason Woodward, Alyssa Teat, Nikolai M. Hay , Matt C. Estep And Zack E. Murrell (2015) "Sixteen Polymorphic Microsateliate Markers For A Federally Threatened Species, Hexastylis Naniflora (Aristolochiaceae), And Co-Occurring Congeners" Applications in Plant Sciences 3 (7) Version of Record Available At www.BioOne.com
Language: English
Date: 2015
Keywords
Aristolochiaceae, Asarum, Hexastylis, Hexastylis , hybrid, microsatellite markers.

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