SHORT TERM TEMPORAL TRENDS IN GENE EXPRESSION IN SENSITIVE AND TOLERANT SOYBEAN GENOTYPES EXPOSED TO OZONE
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Tongji Xing (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
- Advisor
- Howard Neufeld
Abstract: Soybean (Glycine max) is sensitive to O3. My work aimed to identify molecular mechanisms associated with the differential tolerance to O3 among tolerant (Fiskeby III) and sensitive (Mandarin Ottawa) soybean genotypes. Short-term changes in gene expression were investigated using quantitative real-time PCR techniques. Plants were exposed from 9 am to 5 pm at 19.3 + 0.43 ppb or 63.6 + 0.43 ppb O3. A single leaflet was collected just prior to exposure, and at 1, 2, 4, 8, 12, 24, and 48 hours post-exposure, and frozen in liquid N2 until processing. Gas exchange measurements were made on each plant at the end of the exposures to determine if there were changes due to O3 exposure and/or genotype. Photosynthetic rate was not significantly different between the treatments or genotypes, but stomatal conductance was higher in Mandarin than Fiskeby under both O3 conditions. Glutathione reductase 2 (GR2) expression in both genotypes was upregulated under high O3 treatment. Protein D1 (pD1) expression was downregulated in Mandarin but not affected in Fiskeby III, maybe due to the larger gs in Mandarin and subsequently higher dose. An improved understanding of the molecular factors influencing O3 sensitivity could help guide breeders to develop O3 tolerant genotypes.
SHORT TERM TEMPORAL TRENDS IN GENE EXPRESSION IN SENSITIVE AND TOLERANT SOYBEAN GENOTYPES EXPOSED TO OZONE
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Created on 6/7/2016
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Additional Information
- Publication
- Thesis
- Xing, T. (2015) SHORT TERM TEMPORAL TRENDS IN GENE EXPRESSION IN SENSITIVE AND TOLERANT SOYBEAN GENOTYPES EXPOSED TO OZONE. Unpublished master's thesis. Appalachian State University, Boone, NC
- Language: English
- Date: 2015
- Keywords
- Ozone-Sensitivity, Soybean, Glycine-max, Gas-Exchange,
Gene-Regulation