Methanoregula Boonei Gen. Nov., Sp. Nov., An Acidiphilic Methanogen Isolated From An Acidic Peat Bog
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Suzanna Brauer Ph.D., Associate Professor (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
Abstract: A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8T, was isolated from anacidic (pH 4.0–4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures weredimorphic, containing thin rods (0.2–0.3 mm in diameter and 0.8–3.0 mm long) and irregular cocci(0.2–0.8 mm in diameter). The culture utilized H2/CO2 to produce methane but did not utilizeformate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growthconditions were near pH 5.1 and 35 6C. The culture grew in basal medium containing as little as0.43 mM Na+ and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain6A8T is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pureculture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposedthat a new genus and species be established for this organism, Methanoregula boonei gen. nov.,sp. nov. The type strain of Methanoregula boonei is 6A8T (5DSM 21154T 5JCM 14090T).
Methanoregula Boonei Gen. Nov., Sp. Nov., An Acidiphilic Methanogen Isolated From An Acidic Peat Bog
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Additional Information
- Publication
- Bräuer Suzanna L, Cadillo Quiroz H, Ward RJ, Yavitt JB, and Zinder SH.(2011). Methanoregula Boonei Gen. Nov., Sp. Nov., An Acidiphilic Methanogen Isolated From An Acidic Peat Bog." International Journal of Systematic and Evolutionary Microbiology, 61: 45-52. [DOI: 10.1099/ijs.0.021782-0] [ISSN: 1466-5026] Version Of Record Available At www.microbiologyresearch.org
- Language: English
- Date: 2011