Elevated Levels Of The Norspermidine Synthesis Enzyme Nspc Enhance Vibrio Cholerae Biofilm Formation Without Affecting Intracellular Norspermidine Concentrations
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Ece Karatan Ph.D., Associate Professor (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
Abstract: Biofilm formation in Vibrio cholerae is in part regulated by norspermidine, apolyamine synthesized by the enzyme carboxynorspermidine decarboxylase(NspC). The absence of norspermidine in the cell leads to a marked reductionin V. cholerae biofilm formation by an unknown mechanism. In this work, weshow that overexpression of nspC results in large increases in biofilm formationand vps gene expression as well as a significant decrease in motility. Interestingly, increased NspC levels do not lead to increased concentrations of norspermidine in the cell. Our results show that NspC levels inversely regulate biofilm and motility and implicate the presence of an effective feedback mechanism maintaining norspermidine homeostasis in V. cholerae. Moreover, we provide evidence that NspC and the norspermidine sensor protein, NspS, provide independent and distinct inputs into the biofilm regulatory network.
Elevated Levels Of The Norspermidine Synthesis Enzyme Nspc Enhance Vibrio Cholerae Biofilm Formation Without Affecting Intracellular Norspermidine Concentrations
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Additional Information
- Publication
- Parker, Z.M., Pendergraft, S.S., Sobieraj, J., McGinnis, M.M., Karatan, E. (2012). "Elevated Levels Of The Norspermidine Synthesis Enzyme Nspc Enhance Vibrio Cholerae Biofilm Formation Without Affecting Intracellular Norspermidine Concentrations. FEMS Microbiology Letters 329(1):18-27 (ISSN: 0378-1097). [DOI: 10.1111/j.1574-6968.2012.02498.x] Version is Available At www.interscience.wiley.com
- Language: English
- Date: 2012
- Keywords
- norspermidine Synthesis Enzyme, Nspc, vibrio cholerae biofilm, norspermidine concentrations