Role for PPAR-gamma in IL-2 inhibition in T cells by Echinacea-derived undeca-2E-ene-8,10-diynoic acid isobutylamide

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)

Nadja B. Cech, Patricia A. Sullivan Distinguished Professor of Chemistry (Creator)

Ethan W Taylor, Senior Research Professor (Creator)

Institution

The University of North Carolina at Greensboro (UNCG )

Web Site: http://library.uncg.edu/

Abstract: Certain fatty acid amides from Echinacea spp. have demonstrated moderate to high cannabinoid activity. As a result, CB2 activation is currently hypothesized to be the basis of activity for immunomodulation by Echinacea spp. PPAR-gamma, an orphan nuclear receptor and lipid sensor, is known to inhibit IL-2 production and be activated by fatty acid derivatives such as the endocannabinoids. In these investigations, we demonstrate that undeca-2E-ene-8,10-diynoic acid, an Echinacea angustifolia-derived alkylamide lacking affinity for the CB2 receptor, inhibits IL-2 secretion in Jurkat T cells through PPAR-gamma activity at low micromolar concentrations (330 ng/mL). The IL-2 inhibition is reversed by the addition of the selective PPAR? antagonist T0070907. Additionally, we show that that undeca-2-ene-8,10-diynoic acid stimulates 3T3-L1 differentiation, a process dependent on PPAR-gamma activity. These experiments demonstrate that PPAR-gamma is involved in T cell IL-2 inhibition by undeca-2-ene-8,10-diynoic acid and suggest that cytokine modulation by the alkylamides is due to polyvalent activity.

Role for PPAR-gamma in IL-2 inhibition in T cells by Echinacea-derived undeca-2E-ene-8,10-diynoic acid isobutylamide
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Additional Information

Publication

International Immunopharmacology. Volume 9, Issue 11, October 2009, Pages 1260-1264. https://doi.org/10.1016/j.intimp.2009.08.009

Language: English

Date: 2009

Keywords

Echinacea, Alkylamides, Cytokines, Undeca-2E-ene-8,10-diynoic acid isobutylamide, PPAR, IL-2