Selective Detection and Quantification of Modified DNA with Solid-State Nanopores

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Ethan W Taylor, Senior Research Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: We demonstrate a solid-state nanopore assay for the unambiguous discrimination and quantification of modified DNA. Individual streptavidin proteins are employed as high-affinity tags for DNA containing a single biotin moiety. We establish that the rate of translocation events corresponds directly to relative concentration of protein–DNA complexes and use the selectivity of our approach to quantify modified oligonucleotides from among a background of unmodified DNA in solution.

Additional Information

Nano Letters. 2014, 14, 10, 5488–5492.
Language: English
Date: 2014
solid-state nanopores, modified DNA, double-strand DNA, monobiotinylated dsDNA

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