Study of toxicity and uptake of nanoparticles towards understanding biotic-abiotic interactions

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Karshak Kosaraju (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Joseph Starobin

Abstract: With the rapid growth in nanotechnology and tremendous applications the engineered nanomaterials (ENs) offer, there is increase in usage of ENs which increases their likelihood of coming in contact with biological systems which include complex beings like humans and other relatively simpler organism like bacteria and other microorganisms. The interaction between the nanomaterials (NMs) and biological systems includes the formation of protein coronas, particle wrapping, intracellular uptake and bio catalytic processes which could have biocompatible or bio adverse outcomes. Understanding these interactions allows the development of predictive relationships between structure and activity that are mainly determined by NM properties such as size, shape, surface chemistry, aggregation, and surface functionality among many others. This understanding will also provide insight towards the design and development of benign nanomaterials. The overarching goal of this dissertation is to understand the influence of the physicochemical characteristics of the NMs and their influence on their uptake and toxicity when they interact with the biological systems (cells and organs). For this purpose, thoroughly characterized NMs will be exposed to a cellular model, A549 cells (alveolar lung epithelial cells), and a mice model (CD-1 mice) through inhalational administration. The effects of NMs on the in vitro and in vivo models will be evaluated by bio- and immuno-chemical methods to understand toxicity, and a combination of analytical spectroscopic and microscopic tools to study uptake. In vivo toxicity assessment will also be performed by using electrocardiogram (ECG) measurements as a tool to study the effects of inhalation of NMs on cardiac response in mice. Through in vivo studies, a novel non-invasive method, Reserve of Refractoriness (RoR), will be introduced as a tool to study cardiotoxicity.

Additional Information

Language: English
Date: 2015
Characterization, Chemical mechanical planarization, Nanoparticles, Reserve of refractoriness, Toxicity, Uptake
Nanoparticles $x Toxicology
Nanoparticles $x Health aspects
Nanostructured materials $x Toxicology
Nanostructured materials $x Health aspects

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