Brevenal inhibits Pacific ciguatoxin-1B-induced neurosecretion from bovine chromaffin cells
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Daniel G Baden, Director (Creator)
- Andrea J. Bourdelais, Research Associate Professor (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
Abstract: Ciguatoxins and brevetoxins are neurotoxic cyclic polyether compounds produced by dinoflagellates, which are responsible for ciguatera and neurotoxic shellfish poisoning (NSP) respectively. Recently, brevenal, a natural compound was found to specifically inhibit brevetoxin action and to have a beneficial effect in NSP. Considering that brevetoxin and ciguatoxin specifically activate voltage-sensitive Na+ channels through the same binding site, brevenal has therefore a good potential for the treatment of ciguatera. Pacific ciguatoxin-1B (P-CTX-1B) activates voltage-sensitive Na+ channels and promotes an increase in neurotransmitter release believed to underpin the symptoms associated with ciguatera. However, the mechanism through which slow Na+ influx promotes neurosecretion is not fully understood. In the present study, we used chromaffin cells as a model to reconstitute the sequence of events culminating in ciguatoxin-evoked neurosecretion. We show that P-CTX-1B induces a tetrodotoxin-sensitive rise in intracellular Na+, closely followed by an increase in cytosolic Ca2+ responsible for promoting SNARE-dependent catecholamine secretion. Our results reveal that brevenal and ß-naphtoyl-brevetoxin prevent P-CTX-1B secretagogue activity without affecting nicotine or barium-induced catecholamine secretion. Brevenal is therefore a potent inhibitor of ciguatoxin-induced neurotoxic effect and a potential treatment for ciguatera.
Brevenal inhibits Pacific ciguatoxin-1B-induced neurosecretion from bovine chromaffin cells
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Created on 1/1/2009
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Additional Information
- Publication
- Mattei C, Wen PJ, Nguyen-Huu TD, Alvarez M, Benoit E, et al. (2008). Brevenal inhibits Pacific ciguatoxin-1B-induced neurosecretion from bovine chromaffin cells. PLoS ONE 3(10): e3448. doi:10.1371/journal.pone.0003448. © 2008 Mattei et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Language: English
- Date: 2009
- Keywords
- Neurosecretion, Neurotransmitters, Poisonous fishes--Toxicology
- Subjects
- Poisonous fishes--Toxicology
- Neurosecretion
- Neurotransmitters