Studies on the synthesis of threo 2-methylisocitrate

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Nicolas Christopher Coffey (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Jason Reddick

Abstract: Bacillus subtilis is the gold standard organism for Gram-positive bacteria, just as Escherichia coli is it for Gram-negative bacteria. Its genome has been fully sequenced, yet not all of its genes have been characterized. One of these genes, yqiQ, is thought to function in the methylcitric acid cycle as a methylisocitrate lyase, homologous to its E. coli counterpart, prpB. YqiQ would separate the appropriate enantiomer of the threo 2-methylisocitrate pair into succinate and pyruvate. These enantiomorphs, however, are not available commercially, and must be synthesized before characterization of yqiQ is possible. Current literature is lacking in a complete start-to-finish synthesis of threo 2-methylisocitrate, and is discrete regarding the individual reactions required for its synthesis. This thesis explores steps taken towards collecting, merging and improving upon these individual reactions.

Additional Information

Language: English
Date: 2014
Bacillus, Methylisocitrate, Subtilis, Synthesis, Yqiq
Bacillus subtilis

Email this document to