Self-assembled Multifunctional Nanoplexes for Gene Inhibitory Therapy

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Marinella Sandros, Assistant Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:

Abstract: Aim: To enhance the stability of siRNA while improving their therapeutic properties and visualization at the target site, a novel nanoplex system was developed. Materials & Methods: The designed nanoplex system involved functionalizing siRNA with near-infrared quantum dots and loading them into histidylated glycol chitosan (GC-His). Results: Colocalization studies revealed a twofold increase in siRNA uptake after encapsulation with GC-His and nanoparticles were localized in cytoplasm, suggesting that histidine promoted their dissociation from the endosomal membranes. Furthermore, as opposed to siRNAs treated with commercial transfection reagent, siRNAs loaded within GC-His showed a marked reduction (64%) of MDM2 protein expression 24 h after transfection. Conclusion: These findings concur that GC-His/siRNA-quantum dot nanoplexes are promising multifunctional vehicles for gene inhibitory therapy.

Additional Information

Nanomedicine, 6(4), 669-680
Language: English
Date: 2011
glycol chitosan, imaging, N-acetyl-histidine, quantum dots, siRNA

Email this document to