The effects of fortification and refrigerated storage on bioactive proteins in Holder-pasteurized donor human milk

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Maryanne T. Perrin, Assistant Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: Objective: The aim of the study was to assess the total protein, lysozyme, and immunoglobulin A (IgA) content of unfortified and fortified Holder-pasteurized donor human milk (HPDHM) during 96 hours of refrigerated storage. Study Design: HPDHM was prepared in a hospital feeding room and subjected to treatment with 3 different fortifiers: an acidic, bovine-based (F-ACID), a neutral, bovine-based, and a human milk–derived (F-HUM) fortifier. Unfortified HPDHM served as the control (CONTROL). Samples were stored at 4°C, and every 24 hours, a 1-mL aliquot was removed for analysis. Results: At baseline, there was a significant difference in protein (mean, standard deviation) concentration (g/dL) between control (1.3, 0.1) and all other treatments (F-ACID = 2.0, 0.2; neutral, bovine-derived fortifier = 2.2, 0.1; F-HUM = 2.5, 0.1; P < 0.001). Lysozyme and IgA were significantly lower in the F-ACID group (P < 0.001). Lysozyme and IgA were significantly higher in the F-HUM group (P < 0.001). There was no significant effect of storage time (P > 0.9) for all dependent variables. Conclusion: The type of fortifier has a more significant impact on bioactive components in fortified HPDHM than does storage time. Our findings of lack of negative impact of refrigeration storage time on the protein and bioactive components of donor milk strengthen the recent recommendations to extend storage time to 48 hours.

Additional Information

Journal of Pediatric Gastroenterology and Nutrition September 2019 - Volume 69 - Issue 3 - p 370-374 doi: 10.1097/MPG.0000000000002386
Language: English
Date: 2019
bioactives, donor human milk, donor milk, fortified, storage

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