Nanoscale Positioning of Individual DNA Molecules by an Atomic Force Microscope

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Eric Josephs, Assistant Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: Here we report a method to assemble nanoscale DNA structures with single-molecule precision. This assembly is accomplished by performing nanografting in the presence of short, thiolated DNA strands that have been diluted by a positively charged alkanethiol. The expected number of DNA molecules per patch can be modulated by the application of an electric potential to the surface during patterning. Our ability to position individual DNA within a controlled nanoscale environment and observe these molecules in situ will allow us to understand and potentially decouple the heterogeneity caused by the local environment from the intrinsic properties in single-molecule biophysical measurements. Additionally, our approach can potentially be extended to the molecule-by-molecule assembly of larger artificial test structures of nucleic acids or proteins. [The original abstract for this article contains (characters/images) that cannot be displayed here. Please click on the link below to read the full abstract and article.]

Additional Information

Journal of the American Chemical Society, 2010, 132 (30), 10236-38
Language: English
Date: 2010
DNA, nanografting, atomic force microscope

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