A Switchable Surface Enables Detection of Single DNA Hybridization Events with Atomic Force Microscopy

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Eric Josephs, Assistant Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: Here we describe a novel surface that enables direct visualization of the hybridization of single DNA molecules with an unprecedented resolution using atomic force microscopy. The surface consists of single-stranded DNA probes that are covalently anchored to a self-assembled monolayer. The surface satisfies the contradictory requirements for high-resolution imaging and hybridization by switching the DNA-surface interaction between a strong state and a weak state. Our approach opens up unique opportunities in elucidating hybridization at the molecular scale. [The original abstract for this article contains (characters/images) that cannot be displayed here. Please click on the link below to read the full abstract and article.]

Additional Information

Journal of the American Chemical Society, 2013, 135 (17), 6399–6402
Language: English
Date: 2013
DNA, hybridization, atomic force microscopy

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