An enhanced LSPR fiber-optic nanoprobe for ultrasensitive detection of protein biomarkers

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Jianjun Wei, Associate Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: A miniaturized, localized surface plasmon resonance (LSPR)-coupled fiber-optic (FO) nanoprobe is reported as a biosensor that is capable of label-free, sensitive detection of a cancer protein biomarker, free prostate specific antigen (f-PSA). The biosensor is based on the LSPR at the reusable dielectric-metallic hybrid interface with a robust, gold nano-disk array at the fiber end facet that is directly fabricated using EBL and metal lift-off process. The f-PSA has been detected with a mouse anti-human PSA monoclonal antibody (mAb) as a specific receptor linked with a self-assembled monolayer at the LSPR-FO facet surfaces. Experimental investigation and data analysis found near field refractive index (RI) sensitivity at ~226 nm/RIU with current LSPR-FO nanoprobe, and demonstrated the lowest limit of detection (LOD) at 100 fg/mL (~3 fM) of f-PSA in PBS solutions. The control experimentation using 5 mg/mL bovine serum albumin in PBS and nonspecific surface test shows the excellent specificity and selectivity in the detection of f-PSA in PBS. These results present important progress towards a miniaturized, multifunctional fiber-optic technology that integrates informational communication and sensing function for developing a high performance, label-free, point-of-care (POC) device.

Additional Information

Biosensors and Bioelectronics, 2014, 61, 95-101
Language: English
Date: 2014
Fiber optics, Protein biomarker biosensors, Nanofabrication, Au nanodisk array, Localized surface plasmon resonance, Signal transduction

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