Reagentless amperometric biosensor highly sensitive to hydrogen peroxide based on the incorporation of Meldola Blue, fumed-silica and horseradish peroxidase into carbon paste

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Jianjun Wei, Associate Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: A reagentless amperometric sensor highly sensitive to H2O2 has been prepared by incorporating fumed silica, horseradish peroxidase (HRP) and Meldola Blue into carbon paste. The efficient mediating ability to shift electrons between HRP and the carbon paste electrode via Meldola Blue was investigated by cyclic voltammetric and amperometric measurements. Reproducibility, response time, detection limit, selectivity and effects of applied potential, temperature and pH on the response of the sensor are reported. The high sensitivity of the sensor with a detection limit of 0.1 µmol/1 arose from the high efficiency of the bioelectrocatalytic reduction of hydrogen peroxide via HRP and Meldola Blue. The dependence of the Michaelis-Menten constant on the applied potential and the mediator concentration has been investigated and the results are presented.

Additional Information

Fresenius Journal Analytical Chemistry, 1997, 357 (3): 297-301.
Language: English
Date: 1997
Methylene Blue, Fumed Silica, Anal Chim, Direct Electron Transfer, Electron Transfer Mediator

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