Study of nanoscale structural biology using advanced particle beam microscopy

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Adam J. Boseman (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Dennis LaJeunesse

Abstract: This work investigates developmental and structural biology at the nanoscale using current advancements in particle beam microscopy. Typically the examination of micro- and nanoscale features is performed using scanning electron microscopy (SEM), but in order to decrease surface charging, and increase resolution, an obscuring conductive layer is applied to the sample surface. As magnification increases, this layer begins to limit the ability to identify nanoscale surface structures. A new technology, Helium Ion Microscopy (HIM), is used to examine uncoated surface structures on the cuticle of wild type and mutant fruit flies. Corneal nanostructures observed with HIM are further investigated by FIB/SEM to provide detailed three dimensional information about internal events occurring during early structural development. These techniques are also used to reconstruct a mosquito germarium in order to characterize unknown events in early oogenesis. Findings from these studies, and many more like them, will soon unravel many of the mysteries surrounding the world of developmental biology.

Additional Information

Language: English
Date: 2015
Development, FIB/SEM, HIM, Structural Biology
Developmental biology
Scanning electron microscopy

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