Cytotoxicity Screening Matrix Assisted Laser Desorption Ionization Mass Spectrometery for nanoparticle biomarker detection

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Petra C. Wright (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Norman Chiu

Abstract: Toxicology is a broad topic that aims to assess the risk of chemicals on living organisms. It has been acknowledged that everything is toxic to a living organism. What distinguishes a toxin from a remedy depends on the dose 1. Presently, there are toxicity assays that are implemented for human risk assessment studies such as, 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) assay; however, it has been shown that some toxins interfere with these assays. Carbon nanodots, an unknown toxicant has shown to interfere with the LDH assay. Therefore proper methods and techniques must be devised for the analysis of these unknown toxicants to enable the reliable risk assessment for humans. In this study a new method was devised using, hydrogen peroxide and aflatoxin b1 as the known toxicants. The Cytotoxicity Screening Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (cs-MALDI-TOF-MS) technique was implemented as a viable approach to studying nanoparticle toxicity. Previously, Human liver (HepG2) cells were dosed with varying concentrations of these known toxicants and analyzed using cs-MALDI. In this study, Human monocyte (THP-1) cells were dosed with the same concentrations of these known toxicants as the HepG2 and analyzed. This study demonstrates that the two toxicants can be distinguished through the presence and absence of certain peaks using the cs-MALDI method. This method has been developed as a high throughput screening method that can lead to the future identification of biomarkers associated to each toxicant independently.

Additional Information

Language: English
Date: 2014
Cytotoxicity Screening Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry, Toxicity
Toxicity testing
Nanotechnology $x Health aspects
Mass spectrometry

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