Specific recognition of non-denatured nitrite-oxidizing system ofNitrospira moscoviensis by monoclonal antibody Hyb 153-3

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Norman H. Chiu, Assistant Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: The objective of this research project is to develop a rapid molecular method for monitoring nitrification in a wastewater reactor. In the developed method, a monoclonal antibody (Hyb 153-3) was used because it can specifically recognize non-denatured enzymes responsible for nitrite oxidation in Nitrobacter and Nitrospira. The recognition of these enzymes under non-denatured conditions can significantly simplify the procedures of future immunoassays for environmental samples collected from various natural and engineered systems. This paper presents the ability of the selected Hyb 153-3 antibody to recognize the non-denatured form of the nitrite-oxidizing enzyme of Nitrospira in an aqueous phase as well as when the enzyme has been immobilized on a solid surface. Copyright © 2005 Society of Chemical Industry

Additional Information

Language: English
Date: 2006
Hyb 153-3 antibody, autotrophic nitrifying bacteria, biological nutrient removal, chemistry, biochemistry

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