Spatial and temporal translational control of germ cell mRNAs mediated by the eIF4E isoform IFE-1

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)
Andrew J.,Henderson,Melissa A.,Morrison,J. Kaitlin,Hoffma Friday (Creator)
East Carolina University (ECU )
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Abstract: Regulated mRNA translation is vital for germ cells to produce newproteins in the spatial and temporal patterns that drive gametedevelopment. Translational control involves the de-repression ofstored mRNAs and their recruitment by eukaryotic initiation factors(eIFs) to ribosomes. C. elegans expresses five eIF4Es (IFE-1--IFE-5);several have been shown to selectively recruit unique pools of mRNA.Individual IFE knockouts yield unique phenotypes due to inefficienttranslation of certain mRNAs. Here, we identified mRNAspreferentially translated through the germline-specific eIF4E isoformIFE-1. Differential polysome microarray analysis identified 77 mRNAsrecruited by IFE-1. Among the IFE-1-dependent mRNAs are severalrequired for late germ cell differentiation and maturation. Polysomeassociation of gld-1, vab-1, vpr-1, rab-7 and rnp-3 mRNAs relies onIFE-1. Live animal imaging showed IFE-1-dependent selectivity inspatial and temporal translation of germline mRNAs. Altered MAPKactivation in oocytes suggests dual roles for IFE-1, both promotingand suppressing oocyte maturation at different stages. This singleeIF4E isoform exerts positive, selective translational control duringgerm cell differentiation.

Additional Information

Language: English
Date: 2015
mRNA translational control, Oogenesis, C. elegans, Translation state array analysis, TSAA, Protein synthesis, Meiotic maturation

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