Micropepsis pineolensis gen. nov., sp. nov., A Mildly Acidophilic Alphaproteobacterium Isolated From A Poor Fen, And Proposal Of Micropepsaceae fam. nov. Within Micropepsales ord. nov.

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Suzanna Brauer Ph.D., Associate Professor (Creator)
Appalachian State University (ASU )
Web Site: https://library.appstate.edu/

Abstract: An obligately anaerobic, mildly acidophilic, fermentative alphaproteobacterium, designated strain CS4T, was isolated from an acidic, oligotrophic (nutrient poor) poor fen located near Pineola, NC, USA. Cultures contained Gram-negative, slightly curved, non-motile, non-spore-forming, non-prosthecate rods (0.1–0.4 µm in diameter and 0.4–4 µm long, depending, in part, on the growth substrate). Growth optima were 35 °C (range 15–35 °C), pH 5.6 (range of 5.0–6.8), and with 0–50 mM added NaCl (range, 0–100 mM added NaCl). The culture fermented cellobiose, d-glucose, d-mannose, fructose, galactose, glycerol, lactose, maltose, peptone, sucrose, trehalose and xylose. Respiratory growth was not detected. Major fatty acids were C18:1?7c, C19:0 cyclo ?8c, C14:0 and C16:0. The G+C content of the DNA was 61.9±0.3 mol%. The two most closely related species phylogenetically, Rhizomicrobium palustre A48T (AB081581) and Rhizomicrobium electricum Mfc52T (AB365487) shared 94 and 93% SSU rRNA gene sequence identity, respectively, to that of strain CS4T. Lower SSU rRNA gene sequence identities resulted from pairwise comparisons with members of the order ‘Rhizobiales’(85–88%) or Sphingomonadales(85–86%). These findings all support the classification of strain CS4T as representative of a novel genus, family and order of Alphaproteobacteria. The type strain of the species pineolensis within the genus Micropepsis, family Micropepsaceae and order Micropepsales is CS4T (=JCM 30711T=ATCC BAA-2724T).

Additional Information

Harbison, A., Price, L., Flythe, M., & Brauer, S. (2017). Micropepsis pineolensis gen. nov., sp. nov., a mildly acidophilic alphaproteobacterium isolated from a poor fen, and proposal of Micropepsaceae fam. nov. within Micropepsales ord. nov., International Journal of Systematic and Evolutionary Microbiology. Volume 67, Issue 4. https://doi.org/10.1099/ijsem.0.001681. Publisher version of record available at: https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001681
Language: English
Date: 2017
peatland, bacteria, Alphaproteobacteria, Micropepsales

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