Identification of E2F1 as a positive transcriptional regulator for delta-catenin

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)
Sonja Bareiss (Creator)
M. Elizabeth Fini (Creator)
Hyunkyoung Ki (Creator)
Kwonseop Kim (Creator)
Dawei Li (Creator)
Qun Lu (Creator)
Minsoo Oh (Creator)
Tao Wang (Creator)
East Carolina University (ECU )
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Abstract: Delta-Catenin is upregulated in human carcinomas. However little is known about the potential transcriptional factors that regulate delta-catenin expression in cancer. Using a human delta-catenin reporter system we have screened several nuclear signaling modulators to test whether they can affect delta-catenin transcription. Among beta-catenin/LEF-1 Notch1 and E2F1 E2F1 dramatically increased delta-catenin-luciferase activities while beta-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of delta-catenin-luciferase activities induced by E2F1 but did not interact with delta-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of delta-catenin expression is controlled mainly at the transcriptional level. Interestingly the effects of E2F1 on delta-catenin expression were observed only in human cancer cells expressing abundant endogenous delta-catenin. These studies identify E2F1 as a positive transcriptional regulator for delta-catenin but further suggest the presence of strong negative regulator(s) for delta-catenin in prostate cancer cells with minimal endogenous delta-catenin expression. Originally published Biochem Biophys REs Commun Vol. 369 No. 2 May 2008

Additional Information

Biochemical and Biophysical Research Communications. 369:2(May 2008) p. 414-420.
Language: English
Date: 2011
delta-catenin/NPRAP, E2F1, wnt, notch, prostate, catenin, LEF-1, cancer, transcription

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