Modular, Nondegenerate Polyprotein Scaffolds for Atomic Force Spectroscopy

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Eric Josephs, Assistant Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: Atomic force spectroscopy (AFS) is a technique in which a cantilever probe of an atomic force microscope (AFM) is used to directly manipulate and pull on individual protein molecules. In an AFS experiment proteins of interest are deposited onto a surface and, after a protein comes into contact with the probe and is mechanically stretched between the surface and the probe, the forces used to unfold the proteins to an unstructured conformation can be directly determined (reviewed in refs 1-7). Such experiments can provide important insights into the energetics of that protein’s structure and protein folding or unfolding behavior.(8-14)

Additional Information

Biomacromolecules, 2016. 17 (7), 2502-2505
Language: English
Date: 2016
polyproteins, atomic force spectroscopy, codon shuffling

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