Short-term exercise improves myocardial tolerance to in vivo ischemia-reperfusion in the rat

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Andrew Shanely Ph.D, Associate Professor (Creator)
Appalachian State University (ASU )
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Abstract: These experiments examined the independent effects of short-term exercise and heat stress on myocardial responses during in vivo ischemia-reperfusion (I/R). Female Sprague-Dawley rats (4 mo old) were randomly assigned to one of four experimen- tal groups: 1) control, 2) 3 consecutive days of treadmill exercise [60 min/day at 60–70% maximal O2 uptake (V? O2 max)], 3) 5 consecutive days of treadmill exercise (60 min/day at 60–70% V? O2 max), and 4) whole body heat stress (15 min at 42°C). Twenty-four hours after heat stress or exercise, animals were anesthetized and mechanically venti- lated, and the chest was opened by thoracotomy. Coronary occlusion was maintained for 30-min followed by a 30-min period of reperfusion. Compared with control, both heat- stressed animals and exercised animals (3 and 5 days) main- tained higher (P < 0.05) left ventricular developed pressure (LVDP), maximum rate of left venticular pressure develop- ment (+dP/dt), and maximum rate of left ventricular pres- sure decline (-dP/dt) at all measurement periods during both ischemia and reperfusion. No differences existed between heat-stressed and exercise groups in LVDP, +dP/dt, and -dP/dt at any time during ischemia or reperfusion. Both heat stress and exercise resulted in an increase (P < 0.05) in the relative levels of left ventricular heat shock protein 72 (HSP72). Furthermore, exercise (3 and 5 days) increased (P < 0.05) myocardial glutathione levels and manganese superoxide dismutase activity. These data indicate that 3–5 consecutive days of exercise improves myocardial contractile performance during in vivo I/R and that this exercise-induced myocardial protection is associated with an increase in both myocardial HSP72 and cardiac antioxidant defenses

Additional Information

Demirel, Haydar A., Scott K. Powers, Murat A. Zergeroglu, R. Andrew Shanely, Karyn Hamilton, Jeff Coombes, and Hisashi Naito (2001) "Short-term exercise improves myocardial tolerance to in vivo ischemia-reperfusion in the rat" Journal of Applied Physiology #91 pp.2205-2212 Version of Record Available @ (
Language: English
Date: 2001
endurance exercise, heart, reactive oxygen species, heat shock proteins, lipid peroxidation, antioxidant enzymes

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