Enhancing the limit of detection of biomarkers in serum using a SPRi nano-aptasensor

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Stephen Vance (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
James Ryan

Abstract: Surface Plasmon Resonance imaging (SPRi) is a label-free, ultrasensitive detection method for monitoring biomolecular interactions in real-time with high throughput. Diagnostic biomarkers for cancer, cardiovascular disease, and Alzheimer’s disease are often in low abundance in serum, presenting many challenges for their detection. SPRi has great potential as a diagnostic tool because its limit of detection (LOD) for many biomarkers falls in the nanogram per milliliter range, but in order to further enhance its usefulness, its LOD must be reduced to even lower concentrations. We have developed a detection scheme that improves SPRi sensitivity by several orders of magnitude. This increase in sensitivity relies upon the integration of SPRi with nanomaterials and microwave-assisted surface functionalization. This approach makes it possible for the SPRi biosensor to detect C-reactive protein in spiked human serum at concentrations of 5 fg/ml or 45 zeptomole. This scheme was then compared to commercial ELISA kits for the detection of human Growth Hormone, which has a LOD of 1 ng/ml. In order to directly compare the two platforms the antibody sandwich assay was copied in the SPRi scheme and with nanomaterial enhancement, an LOD of 9.2 pg/ml was achieved.

Additional Information

Language: English
Date: 2015
Biomarker, Biosensor, Diagnostics, Nanoparticle, Surface plasmon resonance, Surface plasmon resonance imaging
Surface plasmon resonance
Biochemical markers $x Diagnostic use

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