Characterization Of Acid-Tolerant H2/CO2-Utilizing Methanogenic Enrichment Cultures From An Acidic Peat Bog In New York State

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Suzanna Brauer Ph.D., Associate Professor (Creator)
Appalachian State University (ASU )
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Abstract: Two methanogenic cultures were enriched from acidic peat soil using a growthmedium buffered to c. pH 5. One culture, 6A, was obtained from peat afterincubation with H2/CO2, whereas culture NTAwas derived from a 104 dilution ofuntreated peat into a modified medium. 16S rRNA gene clone libraries from eachculture contained one methanogen and two bacterial sequences. The methanogen16S rRNA gene sequences were 99% identical with each other and belonged to thenovel ‘R-10/Fen cluster’ family of the Methanomicrobiales, whereas their mcrAsequences were 96% identical. One bacterial 16S rRNA gene sequence from culture6A belonged to the Bacteroidetes and showed 99% identity with sequences frommethanogenic enrichments from German and Russian bogs. The other sequencebelonged to the Firmicutes and was identical to a thick rod-shaped citrate-utilizingorganism isolated from culture 6A, the numbers of which decreased when the Ti(III) chelator was switched from citrate to nitrilotriacetate. Bacterial clones fromthe NTA culture clustered in the Delta- and Betaproteobacteria. Both culturescontained thin rods, presumably the methanogens, as the predominant morphotype,and represent a significant advance in characterization of the novel acidiphilic R-10 family methanogens.

Additional Information

Bräuer Suzanna L, Yashiro E, Ueno N*, Yavitt JB, and Zinder SH. (2006). “Characterization Of Acid-Tolerant H2/CO2-Utilizing Methanogenic Enrichment Cultures From An Acidic Peat Bog In New York State.” FEMS Microbiology Ecology, 57(2):206-216. Version Of Record Available At Wiley-Blackwell. (ISSN: 0168-6496). [doi 10.1111/j.1574-6941.2006.00107.x]
Language: English
Date: 2014
methanogenesis, acidiphiles, Methanomicrobiales, peatlands, acidic bogs, bacteria

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