The Effects of Artesunate and Paclitaxel on Human Prostate Cancer Cell Lines

ECSU Author/Contributor (non-ECSU co-authors, if there are any, appear on document)
Juan Fabian, student (Creator)
Elizabeth City State University (ECSU )
Web Site:
Dolapo Adedeji

Abstract: In the present study, the combination of artesunate (ART ) and paclitaxel (PTX) in two humanprostate cancer (PCa) cell lines (PC-3 and LNCaP) was evaluated to investigate the effects onproliferation and apoptosis of PCa cells. The present study explored whether their effects wereassociated with reactive oxygen species (ROS). Half maximal inhibitory concentration (IC50)values were obtained for ART mM (2.126µM) and (0.05µM ) PTX for LNCaP cells and for ART(25.1µM) and (3.98 µM) PTX µM for PC-3 cells at 72 and 120 h. Ratio combination wereassessed by MTT assay using IC50 values at (1:1,1:2, 2:1) to evaluate ratio with least cellviability revealed LNCaP was ART1:PTX1 and PC-3 was ART2:PTX1. Annexin V andpropidium iodide staining was used to investigate apoptosis by flow cytometry. The apoptoticmechanisms of ART + PTX in PCa were investigated by detecting activities of apoptosis,caspase-3/7, and intracellular ROS accumulation. ART + PTX induced PCa apoptosis, ROSaccumulation, caspase 3/7 activity. Taken together, these results indicated that ART + PTXsuppressed PCa cell proliferation in a dose- and time-dependent manner.

Additional Information

Language: English
Date: 2022
artesunate (ART ), paclitaxel (PTX), human prostate cancer (PCa), cell lines

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