Effects of Metformin and Doxorubicin on the Proliferation and Morphology of Androgen-sensitive (LNCaP) and Androgen-insensitive (PC-3) Human Prostate Cancer Cell Lines

ECSU Author/Contributor (non-ECSU co-authors, if there are any, appear on document)
Shenell Brown, student (Creator)
Juan Fabian, student (Creator)
Taylor Pierce, student (Creator)
Dolapo Adedeji , Professor (Contributor)
Gloria Payne, Professor (Contributor)
Elizabeth City State University (ECSU )
Web Site: https://www.ecsu.edu/academics/library/index.html

Abstract: The effects of Metformin (MET) and Doxorubicin (DOX) on human prostate cancer cell lines - LnCAP and PC-3, and morphological changes using Scanning Electron Microscope (SEM) was observed.In vitro anti-proliferative activity of 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was used to assess the effects of metformin and doxorubicin on LnCaP and PC-3 after 72- and 120 – hour exposure. Images of samples with and without the drugs were acquired using SEM. Overall, our study demonstrates that MET and DOX at different concentrations lead to cell death ranging from 42 -87% and 32 – 83% respectively with most effect seen on PC-3. This may offer a promising new therapeutic option for the treatment of metastatic hormone-refractory prostate cancer for patients with diabetes and aid in reducing prostate cancer mortality rate in men.

Additional Information

Language: English
Date: 2021
Metformin (MET), Doxorubicin , prostate cancer, cell lines, Electron Microscope

Email this document to