Mechanobiology Of Mice Cervix: Expression Profile Of Mechano-Related Molecules During Pregnancy

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Jacob Gordon (Creator)
Appalachian State University (ASU )
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Chishimba Nathan Mowa

Abstract: There is a known reciprocation between the chronic exertion of force on tissue and both increased tissue density (e.g. bone) and hypertrophy (e.g. heart). This can also be seen in cervical tissue where the excessive gravitational forces associated with multiple fetal pregnancies promote preterm births. While there is a well-known regulation of cervical remodeling (CR) by sex steroid hormones and growth factors, the role of mechanical force is less appreciated. Using proteome-wide technology, we previously provided evidence for the presence of and alteration in mechano-related signaling molecules in the mouse cervix during pregnancy. Here we profile the expression of select cytoskeletal factors (Filamin A, gelsolin, vimentin, actinin-1, caveolin-1, transgelin, keratin-8, profilin-1) and their associated signaling molecules [Focal adhesion kinase (FAK) and the Rho-GTPases Cdc42, RhoA, and RhoB] in cervices of pregnant mice by real time PCR and confocal immunofluorescent microscopy. Messenger RNA and protein levels increased for each of these 12 factors, except for 3 (keratin-8, profilin-1, RhoA) that decreased during the course of pregnancy, and this corresponded with an increase in gravitational force exerted by the fetus on the cervix. We therefore conclude that size or weight of the growing fetus likely plays a key role in CR through mechano-transduction processes.

Additional Information

Honors Project
Gordon, J. (2018). "Mechanobiology Of Mice Cervix: Expression Profile Of Mechano-Related Molecules During Pregnancy." Unpublished Honors Thesis. Appalachian State University, Boone, NC.
Language: English
Date: 2018
Cervical remodeling, Mice, Mechanotransduction, Pregnancy, Confocal immunofluorescence microscopy

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