Age and attenuation of exercise-induced myocardial HSP72 accumulation

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Andrew Shanely Ph.D, Associate Professor (Creator)
Appalachian State University (ASU )
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Abstract: Overexpression of heat shock protein (HSP)72 is associated with cardioprotection. Hyperthermia-induced HSP72 overexpression is attenuated with senes-cence. While exercise also increases myocardial HSP72 in young animals, it is unknown whether this effect is attenu-ated with aging. Therefore, we investigated the effect of aging on exercise-induced myocardial heat shock factor (HSF)-1 activation and HSP72 expression. Male Fischer-344 rats (6 or 24 mo) were randomized to control, exercise, and hyperthermic groups. Exercise consisted of 2 days of tread-mill running (60 min/day, ~75% maximal oxygen consump-tion). Hyperthermia, 15 min at ~41°C (colonic temperature), was achieved using a temperature-controlled heating blan-ket. Analyses included Western blotting for myocardial HSP72 and HSF-1, electromobility shift assays for HSF-1 activation, and Northern blotting for HSP72 mRNA. Exercise and hyperthermia increased (P ~0.05) myocardial HSP72 in both young (~3.5- and 2.5-fold, respectively) and aged (~3-and 1.5-fold, respectively) animals. Both exercise and hyper-thermic induction of HSP72 was attenuated with age. Myo-cardial HSF-1 protein, HSF-1 activation, and HSP72 mRNA did not differ with age. These data demonstrate that aging is associated with diminished exercise-induced myocardial HSP72 expression. Mechanisms other than HSF-1 activation and transcription of HSP72 mRNA are responsible for this age-related impairment

Additional Information

Haydar A. Demirel, Karyn L. Hamilton, R. Andrew Shanely, Nihal Tu¨ mer, Mary Jo Koroly and Scott K. Powers (2003) "Age and attenuation of exercise-induced myocardial HSP72 accumulation" American Journal of Physiology #285 pp. 1609-1615 Versionf of Record Available at (DOI 10.1152/ajpheart.00982.2002.)
Language: English
Date: 2003
stress proteins, cardioprotection, heart, heat shock protein

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