Improved cardiac performance after ischemia in aged rats supplemented with vitamin E and a-lipoic acid

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Andrew Shanely Ph.D, Associate Professor (Creator)
Appalachian State University (ASU )
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Abstract: The purpose of these experiments was to examine the effects of dietary antioxidant supplementation with vitamin E (VE) and a-lipoic acid (a-LA) on biochemical and physiological responses to in vivo myocardial ischemia-reperfusion (I-R) in aged rats. Male Fischer-334 rats (18 mo old) were assigned to either1) a control diet (CON) or 2)aVEanda-LA supplemented diet (ANTIOX). After a 14-wk feeding period, animals in each group underwent an in vivo I-R protocol (25 min of myocardial ischemia and 15 min of reperfusion). During reperfusion, peak arterial pressure was significantly higher (P,0.05) in ANTIOX animals compared with CON diet animals. I-R resulted in a significant increase ( P,0.05) in myocardial lipid peroxidation in CON diet animals but not in ANTIOX animals. Compared with ANTIOX animals, heart homogenates from CON animals experienced significantly less (P,0.05) oxidative damage when exposed to five different in vitro radical producing systems. These data indicate that dietary supplementation with VE and a-LA protects the aged rat heart from I-R-induced lipid peroxidation by scavenging numerous reactive oxygen species. Importantly, this protection is associated with improved cardiac performance during reperfusion.

Additional Information

Coombes JS, Powers SK, Hamilton KL, Demirel HA, Shanely RA, Zergeroglu AM, Sen CK, Packer L, Ji LL(2000) "Improved cardiac performance after ischemia in aged rats supplemented with vitamin E and a-lipoic acid" Am J Physiol Regulatory Integrative Comp Physiol #279 pp.2149-2155 Version of Record Available @ (
Language: English
Date: 2000
aging, free radicals, lipid peroxidation, myocardial performance

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