Optimization of Autophagic Controls in Human Embryonic Kidney Cells

WCU Author/Contributor (non-WCU co-authors, if there are any, appear on document)
Isabelle Foley (Creator)
Western Carolina University (WCU )
Web Site: http://library.wcu.edu/
Heather Coan

Abstract: Autophagy is a highly conserved and regulated process that plays an essential role in cell survival. It serves as a quality control system that degrades old or damaged material by the autolysosome and recycles it into new materials for the cell. This self-cannibalization process contributes to cellular homeostasis by maintaining a balance between synthesis, degradation, andrecycling, thus preventing the accumulation of toxic substances. Rapamycin and chloroquine are biomaterials that induce or inhibit this response, respectively. Thus, these chemicals represent useful positive and negative controls for studying autophagy. However, studies that use these controls in human cells suggest that cellular responses to their effects are varied. As such, each lab must perform optimization to determine the appropriate dosing concentration and exposure time frame required to induce autophagy. Therefore, this study determined the time and doses required to induce or inhibit autophagy in the Human Embryonic Kidney (HEK) 293 cell line.

Additional Information

Language: English
Date: 2023
autophagy, Human Embryonic Kidney cells

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