Synthesis and characterization of time-resolved lanthanide (III) luminescent probes for potential detection of melanoma skin cancer

WCU Author/Contributor (non-WCU co-authors, if there are any, appear on document)
Brian William Love (Creator)
Western Carolina University (WCU )
Web Site:
Brian Dinkelmeyer

Abstract: The focal point of this research is to continue the development of a time-resolved lanthanide (III) luminescent probe that may aid in early detection of melanoma skin cancer. If melanoma cancer is found at an early stage, it can successfully be taken out by invasive surgery before spreading to other tissues and organs throughout the body. This timely detection could help decrease the mortality rate of melanoma victims. By attaching a lanthanide (III) luminescent tag to a MSH-4 peptide substrate, the detection of melanoma skin cancer could potentially be measured. The design of our target molecule consists of three components. They include: a Eu3+ luminescent complex, a melanocyte stimulating hormone (MSH-4) peptide and a polyethylene glycol spacer molecule. Each component will be independently synthesized and then coupled onto an insoluble support resin via solid phase peptide synthesis. The sequential coupling will allow the indirect attachment of the MSH-4 substrate to the luminescent probe by utilizing a PEGO linker. This separation will ensure that the Eu3+ luminescent tag will not hinder the binding interactions between the MSH-4 substrate and its respective G-protein coupled receptor (GPCR). All components of the probe were characterized by using a combination of 1H NMR, 13C NMR, FT/IR, GC-MS, and HPLC-MS.

Additional Information

Language: English
Date: 2017

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