Detecting DNA Depurination with Solid-State Nanopores

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Michael M. Marshall (Creator)
Ethan W Taylor, Senior Research Professor (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: Among the different types of DNA damage that occur endogenously in the cell, depurination is especially prevalent. These lesions can initiate mutagenesis and have been implicated in a variety of diseases, including cancer. Here, we demonstrate a new approach for the detection of depurination at the single-molecule scale using solid-state nanopores. We induce depurination in short duplex DNA using acidic conditions and observe that the presence of apurinic sites results in significantly slower dynamics during electrokinetic translocation. This procedure may be valuable as a diagnostic for in situ quantification of DNA depurination.

Additional Information

Publication
PLoS ONE 9(7): e101632. https://doi.org/10.1371/journal.pone.0101632
Language: English
Date: 2014
Keywords
deprurination, solid-state nanopores, double stranded DNA, DNA damage

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