Studies on the solid-phase biosynthesis of S-adenosyl-L-methionine

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Stanley Kenneth Mauldin (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Richard Morse

Abstract: The purpose of this study was to develop an affinity chromatography system with S-adenosyl-L-methionine (SAM) as the ligand by a solid-phase biosynthetic reaction between agarose-ATP and L-methionine catalyzed by SAM synthetase. The SAM synthetase in preliminary experiments was a 60- 80 fold purified preparation from E. coli and was provided by R. K. Morse. Because of problems with the results with E. coli SAM synthetase, attempts were made to isolate a purer SAM synthetase preparation from baker's yeast and use this in the solid-phase reaction. The procedure followed for the solid-phase biosynthesis reaction came from unpublished work of R. K. Morse. The method followed for the extraction and purification of SAM synthetase from baker's yeast was a combination of the methods of Mudd and Cantoni (1958), Lombardini et al., (1970), and Chou and Talalay (1972). The SAM synthetase assay method used throughout this study to monitor SAM synthetase activity was developed by Holcomb and Shapiro (1975). The assay measures activity by the separation of 14 CH3-methionine from 14CH3-SAM on Bio-Rex 70 ion exchange resin.

Additional Information

Language: English
Date: 1978

Email this document to